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1.Neuronal nitric oxide synthase: Structure, subcellular localization, regulation, and clinical implications

作者机构:[Zhou, Li; Zhu, Dong-Ya] Nanjing Med Univ, Dept Pharmacol, Sch Pharm, Nanjing 210029, Peoples R Chin...

通讯作者:Zhou, L;Zhu, DY

关键词:nNOS; Nitric oxide; Neurogenesis; Excitotoxicity; PDZ domain;;Depression; Alzheimer's disease; Learn...

摘要:Nitric oxide (NO), a free gaseous signaling molecule, is involved in the regulation of the cardiovascular, nervous and immune system. The neurotransmitter function of nitric oxide is dependent on dynamic regulation of its biosynthetic enzyme, nitric oxide synthase (NOS). There are three types of NOS, neuronal nitric oxide synthase (nNOS), endothelial nitric oxide synthase (eNOS) and inducible nitr...

2.Improvement on Controllable Fabrication of Streptavidin-Modified Three-Layer Core-Shell Fe3O4@SiO2@Au Magnetic Nanocomposites with Low Fluorescence Background

作者机构:[Jiang, Hongrong; Xi, Zhijiang; Liu, Ming; Li, Chuanyan; Li, Zhiyang; Jin, Lian; Deng, Yan; He, Nong...

通讯作者:Jiang, HR;Zeng, X;Xi, ZJ;Liu, M;Li, CY;Li, ZY;Jin, L;Wang, ZF;Deng, Y;He, NY

关键词:Fe3O4@SiO2@Au Magnetic Nanocomposites; Self-Assembling; Seed-Mediated;Growing; Multi-Step Chemical R...

摘要:In present study, we put forward an approach to prepare three-layer core shell Fe3O4@SiO2@Au magnetic nanocomposites via the combination of self-assembling, seed-mediated growing and multi-step chemical reduction. The Fe3O4@SiO2@Au magnetic nanoconnposites were analyzed and characterized by transmission electron microscope (TEM), scanning electronic microscope (SEM), energy dispersive spectrometer...

3.Recent advances in nano scaffolds for bone repair

作者机构:[Yi, Huan; Rehman, Fawad Ur; Zhao, Chunqiu; He, Nongyue] Southeast Univ, Sch Biol Sci & Med Engn, St...

通讯作者:Yi, H;Rehman, FU;Zhao, CQ;Liu, B;He, NY

摘要:Biomedical applications of nanomaterials are exponentially increasing every year due to analogy to various cell receptors, ligands, structural proteins, and genetic materials (that is, DNA). In bone tissue, nanoscale materials can provide scaffold for excellent tissue repair via mechanical stimulation, releasing of various loaded drugs and mediators, 3D scaffold for cell growth and differentiation...

4.Rapid and Sensitive Detection of RNA Viruses Based on Reverse Transcription Loop-Mediated Isothermal Amplification, Magnetic Nanoparticles, and Chemiluminescence

作者机构:[Wang, Jiuhai; Li, Zhiyang] Nanjing Univ, Sch Med, Affiliated Drum Tower Hosp, Dept Clin Lab, Nanjin...

通讯作者:Wang, JH;Lu, P;Yan, JN;Zhang, YF;Huang, LY;Ali, ZS;Liu, B;Li, ZY;He, NY

关键词:RNA Virus; H7N9; RT-LAMR Magnetic Nanoparticles; Chemiluminescence;;Molecular Diagnosis.

摘要:RNA viruses, particularly, the highly pathogenic avian influenza (HPAI) virus, pose serious health concerns, and cause huge economic losses worldwide. Diagnostic tools for the early detection of these deadly RNA viruses are urgently needed to implement treatment and disease control strategies. Conventional reverse transcription polymerase chain reaction (RT-PCR)-based chemiluminescent (RT-PCR-CL) ...

5.The long non-coding RNA H19-derived miR-675 modulates human gastric cancer cell proliferation by targeting tumor suppressor RUNX1

作者机构:[Zhuang, Ming; Gao, Wen; Xu, Jing; Wang, Ping; Shu, Yongqian] Nanjing Med Univ, Affiliated Hosp 1, D...

通讯作者:Zhuang, M;Gao, W;Xu, J;Wang, P;Shu, YQ

关键词:H19; miR-675; RUNX1; Gastric cancer; Cell proliferation

摘要:The IncRNA H19 has been recently shown to be upregulated and play important roles in gastric cancer tumorigenesis. However, the precise molecular mechanism of H19 and its mature product miR-675 in the carcinogenesis of gastric cancer remains unclear. In this study, we found that miR-675 was positively expressed with H19 and was a pivotal mediator in H19-induced gastric cancer cell growth promotion...

6.Exosome Uptake through Clathrin-mediated Endocytosis and Macropinocytosis and Mediating miR-21 Delivery

作者机构:[Tian, Tian; Zhu, Yan-Liang; Zhou, Yue-Yuan; Liang, Gao-Feng; Wang, Yuan-Yuan; Hu, Fei-Hu; Xiao, Zho...

通讯作者:Tian, T;Zhu, YL;Zhou, YY;Liang, GF;Wang, YY;Hu, FH;Xiao, ZD

摘要:Exosomes are nanoscale membrane vesicles secreted from many types of cells. Carrying functional molecules, exosomes transfer information between cells and mediate many physiological and pathological processes. In this report, utilizing selective inhibitors, molecular tools, and specific endocytosis markers, the cellular uptake of PC12 cell-derived exosomes was imaged by high-throughput microscopy ...

7.Genotyping of Pseudomonas aeruginosa Type III Secretion System Using Magnetic Enrichment Multiplex Polymerase Chain Reaction and Chemiluminescence

作者机构:[Tang, Yongjun; Dai, Jianguo] Shenzhen Polytech, Sch Appl Chem & Biotechnol, Shenzhen 518055, People...

通讯作者:Tang, YJ;Ii, B;Dai, JG;Dai, JF;Wang, XH;Si, J;Ali, ZS;Li, TT;He, NY

关键词:Pseudomonas aeruginosa; Type III Secretion System; Magnetic Enrichment;Multiplex PCR; Chemiluminesce...

摘要:The pathologic characteristics and toxicity mechanism of Pseudomonas aeruginosa are different in strains with different Type III secretion system (T3SS) genes. The T3SS gene based genotyping of P. aeruginosa strains is important to understand its virulence and predict the clinical outcomes. In this study, a rapid and automatable method for T3SS genotyping was developed using magnetic enrichment mu...

8.Circular RNA profile in gliomas revealed by identification tool UROBORUS

作者机构:[Song, Xiaofeng] Nanjing Univ Aeronaut & Astronaut, Dept Biomed Engn, Nanjing 210016, Jiangsu, Peopl...

通讯作者:Song, XF;Zhang, NB;Han, P;Moon, BS;Lai, RK;Wang, K;Lu, WG

摘要:Recent evidence suggests that many endogenous circular RNAs (circRNAs) may play roles in biological processes. However, the expression patterns and functions of circRNAs in human diseases are not well understood. Computationally identifying circRNAs from total RNA-seq data is a primary step in studying their expression pattern and biological roles. In this work, we have developed a computational p...

9.Copy Number Variation Analysis Based on Gold Magnetic Nanoparticles and Fluorescence Multiplex Ligation-Dependent Probe Amplification

作者机构:[Yang, Haowen; Liu, Ming; Jiang, Hongrong; Jin, Lian; Deng, Yan; He, Nongyue] Southeast Univ, Dept B...

通讯作者:Yang, HW;Liu, M;Jiang, HR;Zeng, Y;Jin, L;Luan, T;Deng, Y;He, NY;Zhang, G;Zeng, X

关键词:Copy Number Variation; Gold Magnetic Nanoparticles; MLPA; Fluorescence;;Trisomy 21; DSCR3

摘要:A novel strategy for determining relative copy number of target genes was developed in this study based on gold magnetic nanoparticles (GMNPs) and fluorescence multiplex ligation-dependent probe amplification (fMLPA) assay. This method relies on the following stages: (1) amplification of target and reference genes by fMLPA, (2) magnetic capture of fMLPA products on GMNPs, (3) detection of the nano...

10.Highly Selective, Sensitive and Rapid Detection of Escherichia coli O157: H7 Using Duplex PCR and Magnetic Nanoparticle-Based Chemiluminescence Assay

作者机构:[He, Lei; Yang, Haowen; Xiao, Pengfeng; He, Nongyue] Southeast Univ, Sch Biol & Med Engn, State Key ...

通讯作者:He, L;Yang, HW;Xiao, PF;Singh, R;He, NY;Liu, B;Li, ZY

关键词:Magnetic Nanoparticles; Chemiluminescence; E. coli O157: H7; Duplex PCR;;rfbE; fliC

摘要:A novel strategy based on duplex PCR and chemiluminescence (CL) was developed in this study to identify Escherichia coli (E. coli) O157: H7 serotype. The antigen epitopes O157 gene (rfbE) and flagella antigen H7 gene (fliC) were simultaneously amplified and labelled with biotin through duplex PCR. The biotinylated rfbE and fliC amplicons were captured to the surface of oligonucleotide probe-modifi...

11.H3K27 acetylation activated-long non-coding RNA CCAT1 affects cell proliferation and migration by regulating SPRY4 and HOXB13 expression in esophageal squamous cell carcinoma

作者机构:[Zhang, Erbao; He, Xuezhi; Si, Xinxin; De, Wei] Nanjing Med Univ, Dept Biochem & Mol Biol, Nanjing, ...

通讯作者:Zhang, EB;Han, L;Yin, DD;He, XZ;Hong, LZ;Si, XX;Qiu, MT;Xu, TP;De, W;Xu, L;Shu, YQ;Chen, JF

摘要:Recently, long non-coding RNAs (lncRNAs) have been shown to have important regulatory roles in human cancer biology. In our study, we found that lncRNA CCAT1, whose expression is significantly increased and is correlated with outcomes in Esophageal Squamous Cell Carcinoma (ESCC). Consecutive experiments confirmed that H3K27-acetylation could activate expression of colon cancer associated transcrip...

12.SIRT1 antagonizes liver fibrosis by blocking hepatic stellate cell activation in mice

作者机构:[Li, Min; Hong, Wenxuan; Hao, Chenzhi; Li, Luyang; Li, Ping; Xu, Yong] Nanjing Med Univ, Dept Pathop...

通讯作者:Li, M;Hong, WX;Hao, CZ;Li, LY;Wu, DM;Shen, AG;Lu, J;Zheng, YL;Li, P;Xu, Y

关键词:transcriptional regulation; epigenetics; myofibroblast

摘要:Hepatic stellate cells (HSCs) are a major source of fibrogenesis in the liver, contributing to cirrhosis. When activated, HSCs transdifferentiate into myofibroblasts and undergo profound functional alterations paralleling an overhaul of the transcriptome, the mechanism of which remains largely undefined. We investigated the involvement of the class III deacetylase sirtuin [silent information regul...

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