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1.Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

作者机构:[Tan, Yee-Joo; Zeng, Qi] ASTAR, Inst Mol & Cell Biol, Singapore, Singapore.;[Bagniewska-Zadworna, Ag...

通讯作者:Klionsky, DJ;Abdelmohsen, K;Abe, A;Abedin, MJ;Abeliovich, H;Arozena, AA;Adachi, H;Adams, CM;Adams, P...

关键词:autolysosome; autophagosome; chaperone-mediated autophagy; flux; LC3;;lysosome; macroautophagy; phag...

2.Secreted Monocytic miR-150 Enhances Targeted Endothelial Cell Migration

作者机构:[Chen, Xi; Yin, Yuan; Cai, Xing; Zhang, Junfeng] Nanjing Univ, Sch Life Sci, Biomat & Drug Delivery ...

通讯作者:Zhang, YJ;Liu, DQ;Chen, X;Li, J;Li, LM;Bian, Z;Sun, F;Lu, JW;Yin, YA;Cai, X;Sun, Q;Wang, KH;Ba, Y;Wa...

摘要:MicroRNAs (miRNAs) are a class of noncoding RNAs that regulate target gene expression at the posttran-scriptional level. Here, we report that secreted miRNAs can serve as signaling molecules mediating intercellular communication. In human blood cells and cultured THP-1 cells, miR-150 was selectively packaged into microvesicles (MVs) and actively secreted. THP-1-derived MVs can enter and deliver mi...

3.Generation of Gene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos

作者机构:[Niu, Yuyu; Chen, Yongchang; Kang, Yu; Si, Wei; Si, Chenyang; Wang, Hong; Li, Tianqing; Tan, Tao; Pu...

通讯作者:Niu, YY;Shen, B;Cui, YQ;Chen, YC;Wang, JY;Wang, L;Kang, Y;Zhao, XY;Si, W;Li, W;Xiang, AP;Zhou, JK;Gu...

摘要:Monkeys serve as important model species for studying human diseases and developing therapeutic strategies, yet the application of monkeys in biomedical researches has been significantly hindered by the difficulties in producing animals genetically modified at the desired target sites. Here, we first applied the CRISPR/Cas9 system, a versatile tool for editing the genes of different organisms, to ...

4.Lnc RNA HOTAIR functions as a competing endogenous RNA to regulate HER2 expression by sponging miR-331-3p in gastric cancer

作者机构:[Liu, Xiang-hua; Sun, Ming; Zhang, Er-bao; Yin, Dan-dan; Kong, Rong; Xia, Rui; De, Wei] Nanjing Med ...

通讯作者:Liu, XH;Sun, M;Nie, FQ;Ge, YB;Zhang, EB;Yin, DD;Kong, R;Xia, R;Lu, KH;Li, JH;De, W;Wang, KM;Wang, ZX

关键词:Competing endogenous RNA; HER2; HOTAIR; Gastric cancer; Proliferation;and invasion

摘要:Background: Accumulating evidence indicates that the long non-coding RNA HOTAIR plays a critical role in cancer progression and metastasis. However, the overall biological role and clinical significance of HOTAIR in gastric carcinogenesis remains largely unknown. Methods: HOTAIR expression was measured in 78 paired cancerous and noncancerous tissue samples by real-time PCR. The effects of HOTAIR o...

5.Genetic programs in human and mouse early embryos revealed by single-cell RNA sequencing

作者机构:[Xue, Zhigang; Feng, Yun; Liu, Zhenshan; Zeng, Qiao; Cheng, Liming; Sun, Yi E.] Tongji Univ, Sch Med...

通讯作者:Xue, ZG;Huang, K;Cai, CC;Cai, LB;Jiang, CY;Feng, Y;Liu, ZS;Zeng, Q;Cheng, LM;Sun, YE;Liu, JY;Horvath...

摘要:Mammalian pre-implantation development is a complex process involving dramatic changes in the transcriptional architecture(1-4). We report here a comprehensive analysis of transcriptome dynamics from oocyte to morula in both human and mouse embryos, using single-cell RNA sequencing. Based on single-nucleotide variants in human blastomere messenger RNAs and paternal-specific single-nucleotide polym...

6.Sparse whole-genome sequencing identifies two loci for major depressive disorder

作者机构:[Cai, Na; Kretzschmar, Warren; Li, Yihan; Fu, Guangyi; Marchini, Jonathan; Mott, Richard; Flint, Jon...

通讯作者:Cai, N;Bigdeli, TB;Kretzschmar, W;Li, YH;Liang, JQ;Song, L;Hu, JC;Li, QB;Jin, W;Hu, ZF;Wang, GB;Wang...

摘要:Major depressive disorder (MDD), one of the most frequently encountered forms ofmental illness and a leading cause of disability worldwide(1), poses a major challenge to genetic analysis. To date, no robustly replicated genetic loci have been identified(2), despite analysis of more than 9,000 cases(3). Here, using low-coverage whole-genome sequencing of 5,303 Chinese women with recurrent MDD selec...

7.P53-regulated long non-coding RNA TUG1 affects cell proliferation in human non-small cell lung cancer, partly through epigenetically regulating HOXB7 expression

作者机构:[Zhang, E-b; Sun, M.; Kong, R.; Liu, X-h; You, L-h; Xia, R.; De, W.] Nanjing Med Univ, Dept Biochem ...

通讯作者:Zhang, EB;Yin, DD;Sun, M;Kong, R;Liu, XH;You, LH;Han, L;Xia, R;Wang, KM;Yang, JS;De, W;Shu, YQ;Wang,...

关键词:p53; TUG1; proliferation; non-small cell lung cancer; HOXB7

摘要:Recently, a novel class of transcripts, long non-coding RNAs (lncRNAs), is being identified at a rapid pace. These RNAs have critical roles in diverse biological processes, including tumorigenesis. Here we report that taurine-upregulated gene 1 (TUG1), a 7.1-kb lncRNA, recruiting and binding to polycomb repressive complex 2 (PRC2), is generally downregulated in non-small cell lung carcinoma (NSCLC...

8.Long noncoding RNA ANRIL indicates a poor prognosis of gastric cancer and promotes tumor growth by epigenetically silencing of miR-99a/miR-449a

作者机构:[Zhang, Er-bao; Kong, Rong; You, Liang-hui; Sun, Ming; Xia, Rui; De, Wei] Nanjing Med Univ, Dept Bio...

通讯作者:Zhang, EB;Kong, R;Yin, DD;You, LH;Sun, M;Han, L;Xu, TP;Xia, R;Yang, JS;De, W;Chen, JF

关键词:ANRIL; PRC2; miR-99a/miR-449a; proliferation; gastric cancer

摘要:Long noncoding RNAs are involved in diseases including cancer. Here, we reported that ANRIL (CDKN2B-AS1), a 3.8-kb long noncoding RNA, recruiting and binding to PRC2, was generally upregulated in human gastric cancer (GC) tissues. In a cohort of 120 GC patients, the higher expression of ANRIL was significantly correlated with a higher TNM stage (P=0.041) and tumor size (P=0.001). Multivariate anal...

9.Identification of the long non-coding RNA POU3F3 in plasma as a novel biomarker for diagnosis of esophageal squamous cell carcinoma

作者机构:[Tong, Yu-Suo; Liu, Zi-Hao; Yang, Tong-Xin; Shi, Wei-Hong; Xie, Hai-Wei; Lv, Jin; Cao, Xiu-Feng] Nan...

通讯作者:Tong, YS;Wang, XW;Zhou, XL;Liu, ZH;Yang, TX;Shi, WH;Xie, HW;Lv, J;Wu, QQ;Cao, XF

关键词:Esophageal cancer; Long non-coding RNAs; Plasma; Biomarker; Diagnosis

摘要:Background: Recent studies have demonstrated that long non-coding RNAs (lncRNAs) were present in the blood of cancer patients and have shown great potential as powerful and non-invasive tumor markers. However, little is known about the value of lncRNAs in the diagnosis of esophageal squamous cell carcinoma (ESCC). We hypothesized that ESCC-related lncRNAs might be released into the circulation dur...

10.Upregulated IncRNA-UCA1 contributes to progression of hepatocellular carcinoma through inhibition of miR-216b and activation of FGFR1/ERK signaling pathway

作者机构:[Wang, Feng; He, Bang-Shun; Pan, Yu-Qin; Deng, Qi-Wen; Liu, Xian; Wang, Shu-Kui] Nanjing Med Univ, N...

通讯作者:Wang, F;Ying, HQ;He, BS;Pan, YQ;Deng, QW;Sun, HL;Chen, J;Liu, X;Wang, SK

关键词:hepatocellular carcinoma; IncRNA; UCA1; miR-216b; FGFR1

摘要:The long non-coding RNA (lncRNA) urothelial carcinoma-associated 1 (UCA1) has been recently shown to be dysregulated, which plays an important role in the progression of several cancers. However, the biological role and clinical significance of UCA1 in the carcinogenesis of hepatocellular carcinoma (HCC) remain unclear. Herein, we found that UCA1 was aberrantly upregulated in HCC tissues and assoc...

11.Long noncoding RNA PVT1 indicates a poor prognosis of gastric cancer and promotes cell proliferation through epigenetically regulating p15 and p16

作者机构:[Kong, Rong] Northern Jiangsu Peoples Hosp, Clin Med Examinat Ctr, Yangzhou, Jiangsu, Peoples R Chin...

通讯作者:Kong, R;Zhang, EB;Yin, DD;You, LH;Xu, TP;Chen, WM;Xia, R;Wan, L;Sun, M;Wang, ZX;De, W;Zhang, ZH

关键词:PVT1; Cell proliferation; p15; p16; Gastric cancer

摘要:Background: Mounting evidence indicates that long noncoding RNAs (lncRNAs) could play a pivotal role in cancer biology. However, the overall biological role and clinical significance of PVT1 in gastric carcinogenesis remains largely unknown. Methods: Expression of PVT1 was analyzed in 80 GC tissues and cell lines by qRT-PCR. The effect of PVT1 on proliferation was evaluated by MTT and colony forma...

12.The LINK-A lncRNA activates normoxic HIF1 alpha signalling in triple-negative breast cancer

作者机构:[Lin, Aifu; Li, Chunlai; Xing, Zhen; Hu, Qingsong; Liang, Ke; Wang, Shouyu; Zhang, Yanyan; Wei, Yong...

通讯作者:Lin, AF;Li, CL;Xing, Z;Hu, QS;Liang, K;Han, L;Wang, C;Hawke, DH;Wang, SY;Zhang, YY;Wei, YK;Ma, GL;Pa...

摘要:Although long non-coding RNAs (lncRNAs) predominately reside in the nucleus and exert their functions in many biological processes, their potential involvement in cytoplasmic signal transduction remains unexplored. Here, we identify a cytoplasmic lncRNA, LINK-A (long intergenic non-coding RNA for kinase activation), which mediates HB-EGF-triggered, EGFR:GPNMB heterodimer-dependent HIF1 alpha phosp...

13.Complete Meiosis from Embryonic Stem Cell-Derived Germ Cells In Vitro

作者机构:[Zhou, Quan; Yuan, Yan; Liu, Mingxi; Guo, Xuejiang; Sha, Jiahao] Nanjing Med Univ, Dept Histol & Emb...

通讯作者:Zhou, Q;Wang, M;Yuan, Y;Wang, XP;Fu, R;Wan, HF;Xie, MM;Liu, MX;Guo, XJ;Zheng, Y;Feng, GH;Shi, QH;Zha...

摘要:In vitro generation of functional gametes is a promising approach for treating infertility, although faithful replication of meiosis has proven to be a substantial obstacle to deriving haploid gamete cells in culture. Here we report complete in vitro meiosis from embryonic stem cell (ESC)-derived primordial germ cells (PGCLCs). Co-culture of PGCLCs with neonatal testicular somatic cells and sequen...

14.Tumor Evolution of Glioma-Intrinsic Gene Expression Subtypes Associates with Immunological Changes in the Microenvironment

作者机构:[Wang, Qianghu; Hu, Xin; Kim, Hoon; Barthel, Floris; Martinez-Ledesma, Emmanuel; Zheng, Siyuan; Chan...

通讯作者:Wang, QH;Hu, BL;Hu, X;Kim, H;Squatrito, M;Scarpace, L;deCarvalho, AC;Lyu, S;Li, PP;Li, Y;Barthel, F;...

摘要:We leveraged IDH wild-type glioblastomas, derivative neurospheres, and single-cell gene expression profiles to define three tumor-intrinsic transcriptional subtypes designated as proneural, mesenchymal, and classical. Transcriptomic subtype multiplicity correlated with increased intratumoral heterogeneity and presence of tumor microenvironment. In silico cell sorting identified macrophages/microgl...

15.Circular RNA ZNF609 functions as a competitive endogenous RNA to regulate AKT3 expression by sponging miR-150-5p in Hirschsprung's disease

作者机构:[Peng, Lei; Chen, Guanglin; Zhu, Zhongxian; Shen, Ziyang; Du, Chunxia; Zang, Rujin; Su, Yang; Xie, H...

通讯作者:Peng, L;Chen, GL;Zhu, ZX;Shen, ZY;Du, CX;Zang, RJ;Su, Y;Xie, H;Li, HX;Xu, XQ;Xia, YK;Tang, WB

关键词:hirschsprung disease; circular RNA (circRNA); competing endogenous RNAs;(ceRNA); proliferation; migr...

摘要:Research over the past decade suggested critical roles for circular RNAs in the natural growth and disease progression. However, it remains poorly defined whether the circular RNAs participate in Hirschsprung disease (HSCR). Here, we reported that the cir-ZNF609 was down-regulated in HSCR compared with normal bowel tissues. Furthermore, suppression of cir-ZNF609 inhibited the proliferation and mig...

16.A ROR1 HER3 IncRNA signalling axis modulates the Hippo YAP pathway to regulate bone metastasis

作者机构:[Li, Chunlai; Wang, Shouyu; Xing, Zhen; Lin, Aifu; Liang, Ke; Hui, Qingsong; Yao, Jun; Park, Peter K...

通讯作者:Li, CL;Wang, SY;Xing, Z;Lin, AF;Liang, K;Song, J;Hui, QS;Yao, J;Chen, ZY;Park, PK;Hawke, DH;Zhou, JW...

摘要:Bone metastases remain a serious health concern because of limited therapeutic options. Here, we report that crosstalk between ROR1-HER3 and the Hippo-YAP pathway promotes breast cancer bone metastasis in a long noncoding RNA-dependent fashion. Mechanistically, the orphan receptor tyrosine kinase ROR1 phosphorylates HER3 at a previously unidentified site Tyr1307, following neuregulin stimulation, ...

17.Long non-coding RNAs in anti-cancer drug resistance

作者机构:[Chen, Qin-nan; Wei, Chen-chen; Wang, Zhao-xia] Nanjing Med Univ, Affiliated Hosp 2, Dept Oncol, Nan...

通讯作者:Chen, QN;Wei, CC;Wang, ZX;Sun, M

关键词:long non-coding RNAs; cancer; drug resistance; chemotherapy; targets

摘要:Chemotherapy is one of the basic treatments for cancers; however, drug resistance is mainly responsible for the failure of clinical treatment. The mechanism of drug resistance is complicated because of interaction among various factors including drug efflux, DNA damage repair, apoptosis and targets mutation. Long non-coding RNAs ( lncRNAs) have been a focus of research in the field of bioscience, ...

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